Coding

Part:BBa_K833013:Design

Designed by: Julianne Grose   Group: iGEM12_BYUProvo   (2012-10-02)


Lactate sensor derived from the AraF protein of E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 106


Design Notes

Designing primers to incorporate multiple site-directed mutations


Source

This part is a lactate sensor derived from site directed mutagenesis of the E. coli AraF protein.

References